FavorPrep™ Lysozyme/Lyticase Solution Set (100 rxns)

FavorPrep™ Lysozyme/Lyticase Solution Set is designed to pretreat specimens with Gram-positive bacteria & fungal cells in nucleic acid extraction. In the general nucleic acid extraction procedure, proteinase K and guanidine contained lysis buffer is wildly used to lyse tissues and cells, however, the use of proteinase K and guanidine salt is hard to digest the cell wall in both Gram-positive bacteria & fungal cells.
The Gram-positive bacteria contain peptidoglycan layers on the cell wall, which is thicker and hard to digest by proteinases and denaturing agents. The lysozyme is a hydrolytic enzyme available to cleave the β-(1, 4)-glycosidic bonds in the peptidoglycan in the cell wall of Gram-positive bacteria.
The fungi cell contains poly-β-(1, 3)-glucan layers which affect proteinase K and detergent treatment. The Lyticase with β-(1, 3)-glucan laminar hydrolase activity and β-(1, 3)-glucanase activity which able to cleavage the cell wall of yeast and fungus.
FavorPrep™ Lysozyme/Lyticase Mixture provides a convenient way to digest specimens with Gram-positive bacteria & fungal cells in single treatment. It is able to apply in nucleic acid extraction for widely downstream applications, like qPCR, NGS.

Format: Lysozyme Solution and Lyticase Solution

Concentration:

• Lysozyme Solution: 40 mg/ml; ≥1,600,000 U/ml
• Lyticase Solution: ≥ 10,000 U/ml

Applications: Gram-positive bacteria & fungal cells contained specimens.

Operation Time: 15 minutes.