• Tris-NTA Biotin

Tris-NTA Biotin

  • Biotechrabbit
  • BR1001201
  • Na skladě
  • 9 360,00Kč
  • 9 180,00Kč

Dostupné možnosti

- OR -
Tento výrobek má minimální množství 1

Dopravné a balné

Transportní podmínky                                  cena se připočítává k položkám v objednávce

chlazené (CoolPacks)                                      450 Kč bez DPH na zásilky v celkové ceně do 20 000 Kč bez DPH, nad tuto hodnotu je dopravné a balné zdarma

 

Features

  • A complex of three NTA groups ensures high-affinity binding of His-tags
  • Binding affinity is approximately four orders of magnitude higher than monovalent metal ion chelators
  • Protein binding is stochiometric
  • Tris-NTA is available as amino-group or biotin conjugates

Applications

  • Reversible labeling of proteins or cell surfaces
  • Detection and analysis of target molecules
  • Immobilization of proteins, lipids and cells on surfaces
  • Purification and sample preparation of proteins
  • Coupling with microscopic or spectroscopic probes (e.g., fluorophores)

  • Catalog #

    Size

    Package Content

    BR1001201

    100 µg

    100 µl Tris-NTA Biotin

    BR1001202

    1 mg

    1 ml Tris-NTA Biotin

    BR1001203

    1 mg

    1 mg Tris-NTA Biotin (powder)


  • biotechrabbit™ Tris-NTA Biotin for high-affinity His-tag binding.

    His-tags are one of the most commonly used tags for protein expression analysis. Conventional metal ion chelators, such as nitrilotriacetic acid (NTA) and iminodiacetic acid (IDA), bind His-tags with low affinities in the range of 10 µM. The biotechrabbit Tris-NTA complexes three NTA groups that together bind a 6xHis-tag with an affinity that is four orders of magnitude higher (1 nM) than is possible with conventional chelators. The binding of His-tags is stoichiometric, and single-molecule detection is possible. Binding is reversible: bound His-tags can be released with imidazole or ethylenediaminetetraacetic acid (EDTA).
    biotechrabbit Tris-NTA is available in amino-group-conjugated and biotinylated forms and can be used in a large range of applications, including protein detection and marking, protein, lipid or cell coupling to surfaces, protein purification and reversible protein modification.