Hot Start PCR Master Mix, 2x
- Biotechrabbit
- BR0200200
- In Stock
-
2,964.00Kč
Available Options
Storage temperature: Dry ice
Features
- Highest PCR sensitivity without prolonged reactivation
- Optimized PCR Master Mix for minimal hands-on and fast setup
- Exceptionally pure Hot Start Taq DNA Polymerase and highest quality dNTPs
- Highest PCR sensitivity without prolonged reactivation
- Optimized PCR Master Mix for minimal hands-on and fast setup
- Exceptionally pure Hot Start Taq DNA Polymerase and highest quality dNTPs
Applications
- High-specificity and high-throughput hot-start PCR up to 3 kb
- Amplification of low-copy-number targets
- TA cloning
- High-specificity and high-throughput hot-start PCR up to 3 kb
- Amplification of low-copy-number targets
- TA cloning
Catalog # Size Package Content
BR0200205 200 reactions of 50 µl 4 × 1.25 ml Hot-Start PCR Master Mix
BR0200206 1000 reactions of 50 µl 20 × 1.25 ml Hot-Start PCR Master Mix
BR0200207 4000 reactions of 50 µl 4 kits BR0200206 Hot-Start PCR Master Mix (1000 rxn each)
biotechrabbit™ Hot Start PCR Master Mix is a perfect choice for a fast reaction setup that reduces the time required for calculation and pipetting and eliminates the need for buffer optimization. It is designed for low-background, high-throughput PCR of 0.2–3 kb DNA targets.
The 2x Hot Start PCR Master Mix contains pure biotechrabbit Hot Start Taq DNA Polymerase, extremely high-quality dNTPs and optimized PCR buffer; thus, only template, PCR primers and PCR-grade water are added.
The Hot Start Taq DNA Polymerase is inactive during reaction setup due to the bound antibody, which is quickly released at elevated temperatures, ensuring the enzyme is active only during PCR. There is no need for prolonged heating or denaturation steps. The hot start minimizes primer–dimers and mispriming.
Info: Recommended annealing temperature is 2°C above Tm of primers or use gradient PCR to optimize the annealing temperature.
| Catalog # | Size | Package Content | ||
|---|---|---|---|---|
| BR0200205 | 200 reactions of 50 µl | 4 × 1.25 ml Hot-Start PCR Master Mix | ||
| BR0200206 | 1000 reactions of 50 µl | 20 × 1.25 ml Hot-Start PCR Master Mix | ||
| BR0200207 | 4000 reactions of 50 µl | 4 kits BR0200206 Hot-Start PCR Master Mix (1000 rxn each) |
biotechrabbit™ Hot Start PCR Master Mix is a perfect choice for a fast reaction setup that reduces the time required for calculation and pipetting and eliminates the need for buffer optimization. It is designed for low-background, high-throughput PCR of 0.2–3 kb DNA targets. The 2x Hot Start PCR Master Mix contains pure biotechrabbit Hot Start Taq DNA Polymerase, extremely high-quality dNTPs and optimized PCR buffer; thus, only template, PCR primers and PCR-grade water are added. The Hot Start Taq DNA Polymerase is inactive during reaction setup due to the bound antibody, which is quickly released at elevated temperatures, ensuring the enzyme is active only during PCR. There is no need for prolonged heating or denaturation steps. The hot start minimizes primer–dimers and mispriming. Info: Recommended annealing temperature is 2°C above Tm of primers or use gradient PCR to optimize the annealing temperature. |