• Hot Start PCR Master Mix, 2x

Hot Start PCR Master Mix, 2x

  • Biotechrabbit
  • BR0200200
  • In Stock
  • 3,692.00Kč

Available Options

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This product has a minimum quantity of 1
Features
  • Highest PCR specificity and sensitivity without prolonged reactivation
  • Optimized PCR Master Mix for minimal hands-on and fast setup
  • Exceptionally pure Hot Start Taq DNA Polymerase and highest quality dNTPs

Applications
  • High specificity and high throughput hot-start PCR up to 5kb
  • Amplification of low-copy-number targets
  • TA cloning

  • Catalog #

    Size

    Package Content

    BR0200201

    100 rxn of 50 µl

    2 × 1.25 ml Hot Start PCR Master Mix 
    500 µl 5× PCR Enhancer

    BR0200202

    500 rxn of 50 µl

    10 × 1.25 ml Hot Start PCR Master Mix 
    1.5 ml 5× PCR Enhancer

    BR0200204

    2000 rxn of 50 µl

    40 × 1.25 ml Hot Start PCR Master Mix 
    5 × 1.5 ml 5× PCR Enhancer


  • biotechrabbit Hot Start PCR Master Mix is a perfect choice for a fast reaction setup that reduces the time required for calculation and pipetting and eliminates the need for buffer optimization. It is designed for lowbackground, high-throughput PCR of 0.2–5 kb DNA targets.
     
    The 2x Hot Start PCR Master Mix contains pure biotechrabbit Hot Start Taq DNA Polymerase, extremely high-quality dNTPs and optimized PCR buffer; thus, only template, PCR primers and PCR-grade water are added.
     
    The Hot Start Taq DNA Polymerase is inactive during reaction setup due to the bound antibody, which is quickly released at elevated temperatures, ensuring the enzyme is active only during PCR. There is no need for prolonged heating or denaturation steps. The hot start minimizes primer–dimers and miss-priming.
     
    The optional use of 5× PCR Enhancer improves PCR results in many cases, including impure template or low template abundance.