• Green Hot Start Taq DNA Polymerase, 5 U/µl

Green Hot Start Taq DNA Polymerase, 5 U/µl

  • Biotechrabbit
  • BR0200700
  • In Stock
  • 2,990.00Kč

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Features
  • Green Reaction Buffer formulation allows for direct loading on the gel right after the PCR
  • High PCR specificity and sensitivity
  • Hot Start Taq DNA Polymerase for demanding sensitive PCR applications and high yields
  • High value for a fair price

Applications
  • High throughput PCR for an immediate gel analysis
  • High specificity Hot Start PCR up to 5kb 
  • Amplification of low copy number targets
  • RT-PCR, TA cloning

  • Catalog #

    Size

    Package Content

    BR0200701

    100 U

    20 µl Hot Start Taq DNA Polymerase
    1.5 ml 5× Green Reaction Buffer
    1.5 ml 50 mM MgCl2

    BR0200702

    500 U

    100 µl Hot Start Taq DNA Polymerase
    4 × 1.5 ml 5× Green Reaction Buffer
    1.5 ml 50 mM MgCl2

    BR0200703

    2500 U

    5 × 100 µl Hot Start Taq DNA Polymerase
    20 × 1.5 ml 5× Green Reaction Buffer
    5 × 1.5 ml 50 mM MgCl2


  • biotechrabbit Green Hot Start Taq DNA Polymerase is a first-choice hot-start PCR enzyme for all demanding PCR applications. The enzyme ensures high product yields with low background; without primer–dimer formation and nonspecific priming, allowing direct electrophoresis without the need to add loading buffer and ensuring high product yields from various templates.

    The Hot Start Taq Polymerase is inactive during reaction setup due to the bound antibody which is quickly released at elevated temperatures, ensuring the enzyme is active only during PCR. There is no need for prolonged heating or denaturation steps.

    The 5x Reaction Buffer of Green Taq DNA Polymerase is recommended for any amplification reaction that will be visualized by agarose gel electrophoresis and ethidium bromide staining. The 5x Reaction Buffer contains two dyes (blue and yellow) that separate during electrophoresis, allowing the migration progress to be monitored. Reactions assembled with 5x Reaction Buffer have sufficient density for direct loading onto agarose gels.

    The PCR specificity can be further increased in some cases by addition of the supplied 5x PCR Booster. The optional use of 5x PCR Booster improves PCR results in many cases, including impure template or low template amounts.

    Taq DNA polymerase is a thermostable highly processive 5'→3' DNA polymerase that has low 5'→3' exonuclease activity and lacks 3'→5' exonuclease (proofreading) activity. The enzyme also exhibits deoxyribonucleotidyl transferase activity that results in the addition of an extra A overhang at the 3' ends of PCR products, allowing easy cloning of PCR products into vectors with T overhangs.

    Note: The dyes absorb between 225 – 300 nm, making standard A260 readings to determine DNA concentration unreliable.