RTS 9000 E. coli HY Kit

One RTS 9000 reaction produces ten times more total protein yield (up to 50 mg) than an RTS 500 reaction. This higher total yield is achieved by increasing the reaction volume by a factor of ten, while the productivity per milliliter of lysate in the RTS 500 E. coli HY and RTS 9000 E. coli HY reactions is similar.

The RTS 9000 Reaction Device is composed of a reaction compartment and a feeding compartment. The 10 ml reaction compartment, where the coupled transcription/translation reaction takes place, is separated from the 100 ml feeding compartment by a semipermeable membrane (cut-off 10 kDa). The plasmid that contains the gene of interest serves as a template for the transcription. An optimized, biochemically enhanced High-Yield (HY) E. coli lysate contains tRNAs, ribosomes, enzymes, factors, and other necessary components required for highly efficient translation. The feeding compartment continuously supplies ions, energy substrates, nucleotides, and amino acids through the semipermeable membrane. Simultaneously, by-products that may inhibit the reaction are diluted through the same membrane into the feeding compartment. This continuous exchange of substrates and by-products across the two semipermeable membranes (CECF technology) allows protein synthesis to continue for up to 24 hours.