Whole Blood RNA Mini Kit

RNA extraction

The FavorPrep™ Whole Blood RNA Mini Kit is designed for rapid and efficient isolation of total RNA from frozen and fresh whole blood samples. The kit uses a chaotropic salt-based formula for cell lysis, RNase inactivation and RNA binding (>200 nt, e.g., 18S, 28S RNA, pri-miRNA) to silica membrane. The optional on-column DNase I digestion technique offers a convenient DNA removal method. The high-purity RNA is eluted from the membrane by using a low-ionic-strength buffer. This extracted total RNA is ready-to-use for downstream applications, such as real-time RT-PCR, cDNA synthesis, Northern blotting, primer extension, miRNA selection, etc.

Format: Mini spin column (RNA Binding Column)

Principle: Silica-membrane technology/chaotropic salt binding

Sample Size: 200~400 µl whole blood

Size of Isolated RNA: >200 nucleotides

Typical RNA Yield: 5~7 µg/ 1 ml whole blood

Operation Time: ≤50 mins

Binding Capacity: ≤100 µg RNA/column

Column Applicability: Centrifugation and vacuum

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