ProRT Reverse Transcriptase (MMLV-RT, Thermostable RT H-)
- Protean s.r.o.
- 1972
- In Stock
-
2,983.00Kč
Available Options
Type | Recombinant |
Source | E. coli |
Species | MMLV |
Tag | His6 |
Form | Liquid |
Purity | >95% by SDS PAGE |
Shipping | Ice pack |
Introduction
Moloney Murine Leukemia Virus encoded sequence of reverse transcriptase was in silico optimized and produced in E.coli strain.
Description
Higly active recombinant enzyme with an increase thermal stability. RNAse H-minus fragment of reverse transcriptase suitable for all molecular techniques requiring reliable reverse transcriptase activity, such as RT-PCR and RT-LAMP assays. An increased thermal stability of this particular enzyme allow coupled reverse transcription at elevated temperatures up to 60°C, which results in an increased sensitivity and specificity of various detection techniques. The concentration is 200 U/ul.
Application
RT-LAMP, RT-PCR, RT-qPCR
Purification method
Affinity chromatography. The enzymes supplied by Protean Ltd. are manufactured in certified environment under ISO 9001 and ISO 13485 international standards, which fully qualifies them for a use in downstream GMP certified processes.
Formulation
Storage buffer: 10mM Tris (pH 7,5), 50mM KCl, 1mM DTT, 0,1mM EDTA, 0,1% Triton, 50% glycerol. 10x Reaction Buffer: Proprietary optimized buffer.
Specificity
RNA
Storage
Store at -20°C
For research use only. Not for use in diagnostic procedures.
Manufactured in ISO 9001 and ISO 13485 certified laboratory.