• T4 DNA Ligase Rapid, 600 U/µl

T4 DNA Ligase Rapid, 600 U/µl

  • Biotechrabbit
  • BR1100300
  • In Stock
  • 2,886.00Kč
  • 2,831.00Kč

Available Options

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This product has a minimum quantity of 1

Storage temperature: Dry ice

Features

  • Exceptionally pure high concentration T4 DNA Ligase for demanding applications
  • Rapid ligation
  • Supplied with two buffers for fast and usual ligation protocols
  • High value for a fair price

Applications

  • Blunt and sticky end DNA Ligation for cloning
  • Joining of linkers or adaptors to ds DNA
  • Self-circularization of linear DNA

  • Catalog #

    Size

    Package Content

    BR1100301

    60000 U | 100 rxn

    100 µl T4 DNA Ligase Rapid
    1.5 ml 2× Rapid Ligation Buffer
    500 µl 10× Ligation Buffer

    BR1100302

    180000 U | 300 rxn

    3 × 100 µl T4 DNA Ligase Rapid
    3 × 1.5 ml 2× Rapid Ligation Buffer
    3 × 500 µl 10× Ligation Buffer


  • The biotechrabbit T4 DNA Ligase, Rapid, is an exceptionally pure highly concentrated ligase for applications where high concentrations of the enzyme are required. It is especially recommended for fast ligations.

    T4 DNA Ligase, Rapid is supplied with two buffers – the common 10x ligation buffer for usual 1 hour or overnight ligations and the 2x Rapid Ligation Buffer containing PEG for fast 5- 10 minutes ligations or ligation of low concentration or blunt ended DNA.

    The 10X Ligation Buffer does not contain PEG and is compatible with standard ligation protocols which do not specify the use of a rapid/fast/quick format buffer.

    The T4 DNA Ligase, Rapid in combination with the 2X Rapid Ligation buffer greatly stimulates the rate and efficiency blunt-end ligation, therefore long incubations (>10 minutes) are not recommended as they reduce the transformation efficiency of ligation products.

    T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids (1).

    The enzyme is purified from a recombinant E. coli strain carrying the cloned T4 DNA Ligase gene.