• Green Taq DNA Polymerase, 5 U/µl

Green Taq DNA Polymerase, 5 U/µl

  • Biotechrabbit
  • BR0100300
  • In Stock
  • 2,730.00Kč

Available Options

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This product has a minimum quantity of 1

Features

  • Green Reaction Buffer formulation allows for direct loading on the gel right after the PCR
  • High product yields and robustness in a wide application range
  • Exceptionally pure Taq DNA Polymerase for both routine and demanding PCR applications
  • High value for a fair price

Applications

  • High throughput PCR for an immediate gel analysis
  • Routine PCR up to 5kb, TA cloning

  • Catalog #

    Size

    Package Content

    BR0100301

    100 U

    20 µl Taq DNA Polymerase
    2 × 1.5 ml 5× Green Reaction Buffer
    1.5 ml 50 mM MgCl2

    BR0100302

    500 U

    100 µl Taq DNA Polymerase
    4 × 1.5 ml 5× Green Reaction Buffer
    1.5 ml 50 mM MgCl2

    BR0100303

    2500 U

    5 × 100 µl Taq DNA Polymerase
    20 × 1.5 ml 5× Green Reaction Buffer
    5 × 1.5 ml 50 mM MgCl2


  • The biotechrabbit Green Taq DNA Polymerase is a first choice thermostable DNA polymerase for routine PCR applications allowing direct electrophoresis without the need to add loading buffer and ensuring high product yields from various templates.

    The 5x Green Taq Reaction Buffer is recommended for any amplification reaction that will be visualized by agarose gel electrophoresis and ethidium bromide staining. The Buffer contains two dyes (blue and yellow) that separate during electrophoresis to monitor migration progress. Reactions assembled with Green Reaction Buffer have sufficient density for direct loading onto agarose gels.

    The dyes absorb between 225–300nm, making standard A260 readings to determine DNA concentration unreliable.

    Taq DNA polymerase is thermostable highly processive 5´ → 3´ DNA polymerase that has low 5´ → 3´ exonuclease activity and lacks 3´ → 5´ exonuclease (proofreading) activity. The enzyme also exhibits deoxynucleotidyl transferase activity what results in the addition of extra A overhang at the 3'-ends of PCR products allowing easy cloning of PCR products into vectors with T overhangs.