• Tris-NTA Amine

Tris-NTA Amine

  • Biotechrabbit
  • BR1001101
  • In Stock
  • 5,590.00Kč
  • 5,483.00Kč

Available Options

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Features

  • A complex of three NTA groups ensures high-affinity binding of His-tags
  • Binding affinity is approximately four orders of magnitude higher than monovalent metal ion chelators
  • Protein binding is stochiometric
  • Tris-NTA is available as amino-group or biotin conjugates

Applications

  • Reversible labeling of proteins or cell surfaces
  • Detection and analysis of target molecules
  • Immobilization of proteins, lipids and cells on surfaces
  • Purification and sample preparation of proteins
  • Coupling with microscopic or spectroscopic probes (e.g., fluorophores)

  • Catalog #

    Size

    Package Content

    BR1001101

    100 µg

    100 µl Tris-NTA Amine

    BR1001102

    1 mg

    1 ml Tris-NTA Amine

    BR1001103

    1 mg

    1 mg Tris-NTA Amine (powder)


  • biotechrabbit™ Tris-NTA Amine for high-affinity His-tag binding.

    His-tags are one of the most commonly used tags for protein expression analysis. Conventional metal ion chelators, such as nitrilotriacetic acid (NTA) and iminodiacetic acid (IDA), bind His-tags with low affinities in the range of 10 µM. The biotechrabbit Tris-NTA complexes three NTA groups that together bind a 6xHis-tag with an affinity that is four orders of magnitude higher (1 nM) than is possible with conventional chelators. The binding of His-tags is stoichiometric, and single-molecule detection is possible. Binding is reversible: bound His-tags can be released with imidazole or ethylenediaminetetraacetic acid (EDTA).
    biotechrabbit Tris-NTA is available in amino-group-conjugated and biotinylated forms and can be used in a large range of applications, including protein detection and marking, protein, lipid or cell coupling to surfaces, protein purification and reversible protein modification.