Protein Synthesis for Site-Directed Labeling
Proteins with unique functional groups such as affinity labels, fluorescence moieties or drug conjugates offer high potential in many biotechnological or biomedical applications.
An attractive alternative to known conventional posttranslational methods of protein modification is the site-directed incorporation of unnatural amino acids. In a specialized cell-free system, amino acids labeled with dyes or chemoselective reactive amino acids are incorporated stoichiometrically at defined positions of the expressed protein. Such homogenous site specific-modified proteins are the basis for a broad range of downstream applications.