Hot Start PCR Mix, lyophilized, 2x

Dopravné a balné

Transportní podmínky                                

pokojová teplota (RT)                                     

Features

• Room-temperature stable enzymes and mixes
• Exceptionally pure Taq DNA Polymerase
• Hot-start for high PCR specificity and sensitivity
• Optimized Master Mix for fast setup

Applications

• Ambient shipment and room-temperature storage
• Routine and demanding PCR applications
• PCR amplification up to 3 kb
• TA cloning

Purchase 2X Hot-Start PCR Master Mix, lyophilized

Availability: In stock Bulk requests please inquire at oem@biotechrabbit.com

Catalog #	Size	Package Content
BR0201103	200 reactions of 50 µl	4 × 2X Lyo Hot-Start PCR Master Mix 4 × 1.25 ml PCR Master Mix Reconstitution Buffer

iotechrabbit™ Lyo Hot Start PCR Master Mix is a freeze dried version of the well-established liquid equivalent. The stabilized format allows shipment and storage without cooling. The Master Mix is a perfect choice for a fast reaction setup that reduces the time required for calculation and pipetting and eliminates the need for buffer optimization. It is designed for low-background, high-throughput PCR of 0.2–3 kb DNA targets. The 2x Hot Start PCR Master Mix contains pure biotechrabbit Hot Start Taq DNA Polymerase, extremely high-quality dNTPs and optimized PCR buffer; thus, only template, PCR primers and PCR-grade water are added. The Hot Start Taq DNA Polymerase is inactive during reaction setup due to the bound antibody, which is quickly released at elevated temperatures, ensuring the enzyme is active only during PCR. There is no need for prolonged heating or denaturation steps. The hot start minimizes primer–dimers and mispriming.   Info: Recommended annealing temperature is 2°C above primer Tm (use gradient PCR to optimize the annealing temperature).