• Taq DNA Ligase (Thermostable Ligase, Ampligase)

Taq DNA Ligase (Thermostable Ligase, Ampligase)

  • 2 435,00 Kč

- NEBO -

2 KU

Type Recombinant

Source E. coli

Taq Ligase is thermostable DNA ligase from the thermophilic bacterium Thermus aquaticus cloned to E.

coli cells. This enzyme is able to be stable and active at much higher temperatures than conventional

DNA ligases and could be used at PCR conditions. However, it cannot be used to replace T4 DNA Ligase in

most cloning methods due to insufficient activity at low temperatures where 2- and 4-base cohesive ends

form stable duplexes or blunt ends. Taq Ligase is a 74 kDa protein catalyzing the NAD-dependent ligation

of adjacent 3-hydroxyl and 5-phosphate termini in duplex DNA structures. It does not exhibit activity on

blunt ends or RNA substrates. This enzyme is active in a variety of DNA polymerase buffers within a pH

range of 7-8.

Manufactured in ISO 9001 and ISO 13485 certified laboratory.

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