• RTS Linear Template Kit Plus

RTS Linear Template Kit Plus

  • Biotechrabbit
  • BR1402401
  • Na skladě
  • 8 970,00Kč
  • 8 798,00Kč

- OR -
Tento výrobek má minimální množství 1

Dopravné a balné

Transportní podmínky                                  cena se připočítává k položkám v objednávce

mražené na suchém ledu (dry ice)                   890 Kč bez DPH na zásilky v celkové ceně do 45 000 Kč bez DPH, nad tuto hodnotu je dopravné a balné zdarma


Features

  • Efficient in vitro expression (E. coli and insect kits)
  • Primers for affinity tags (His6-tag /Strep-tag II)
  • Signal peptide sequence primers for expression translocation into microsomes (insect systems)

Applications

  • Fast generation of multiple template constructs
  • Accelerated screening of protein expression templates and conditions


  • Catalog #

    Size

    Package Content

    BR1402401

    20 PCRs of 25 µl

    PCR reagents for linear template generation


  • The RTS Linear Template Kit Plus accelerates the generation of multiple templates for parallel protein expression. Constructs are suitable for expression in E. coli and insect cell-free lysates. Due to compatible restriction sites in the adapter primers, products can be easily cloned into pIX3.0 vectors for large-scale in vitro expression.

    The kit contains primers providing regulatory elements required for optimal transcription and translation in cell-free expression systems. Specially designed 5'-untranslated regions reduce the formation of secondary structure in the translation initiation region, one of the most common causes of low expression rates. A His-tag or Strep-tag II can be added to either terminus, greatly simplifying protein purification and detection.

    A signal sequence enables expression of functional proteins containing disulfide-bonds or membrane proteins in RTS 100 Insect Membrane or Disulfide kits. A signal peptide can be introduced using the N-terminal primer, which contains a melittin sequence. This enables protein translocation into microsomal vesicles derived from insect cell endoplasmic reticulum.

    (former FastLane product codes: RiNA C-1223-20)