• Long Range PCR Master Mix, 2x

Long Range PCR Master Mix, 2x

  • Biotechrabbit
  • BR0300400
  • Na skladě
  • 3 250,00Kč
  • 3 188,00Kč

Dostupné možnosti

- OR -
Tento výrobek má minimální množství 1

Dopravné a balné

Transportní podmínky                                  cena se připočítává k položkám v objednávce

mražené na suchém ledu (dry ice)                   890 Kč bez DPH na zásilky v celkové ceně do 45 000 Kč bez DPH, nad tuto hodnotu je dopravné a balné zdarma

Features
  • Optimized Long Range PCR Master Mix for minimal hands-on and fast setup
  • Mix of pure polymerases and highest quality dNTPs for high yield and short cycle times
  • Increased fidelity for accurate amplification of GC-rich templatess
Applications
  • High throughput, long-range PCR up to 40 kb
  • Amplification of GC-rich templates

  • Catalog #

    Size

    Package Content

    BR0300401

    100 rxn of 50 µl

    2 × 1.25 ml Long Range PCR Master Mix
    500 µl 5× PCR Enhancer

    BR0300402

    500 rxn of 50 µl

    10 × 1.25 ml Long Range PCR Master Mix
    1.5 ml 5× PCR Enhancer


  • The biotechrabbit Long Range PCR Master Mix is a perfect choice for a fast reaction set up for long PCR without the need for calculation, multiple pipetting steps and buffer optimization. It is designed for PCR amplifications of up to 30kb size DNA targets. The Master Mix works well on GC rich templates and amplifies DNA with a higher fidelity than that of Taq DNA Polymerase.

    The Long Range PCR Master Mix contains a blend of thermophilic polymerases, highest quality dNTPs and optimal PCR reaction buffer components, thus only the template and PCR primers have to be added. The final reaction volume should be reached with the pure PCR grade water.

    For the most demanding applications the supplied PCR additive 5x PCR Helper can be used. It is not necessary to use for every reaction, but in many cases it helps to achieve even better results with templates having high GC content and complex structures.

    Long Range PCR Master Mix produces a mixture of A tailed and blunt ended PCR products, it is advisable to blunt them before cloning into the blunt ended vector.