• RTS 100 E. coli Disulfide Kit

RTS 100 E. coli Disulfide Kit

  • Biotechrabbit
  • BR1400401
  • Na skladě
  • 21 450,00Kč
  • 21 038,00Kč

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mražené na suchém ledu (dry ice)  -70 °C                 890 Kč bez DPH na zásilky v celkové ceně do 45 000 Kč bez DPH, nad tuto hodnotu je dopravné a balné zdarma


Features

  • Express disulfide-bonded proteins in functional form due to the optimized lysate and reaction conditions
  • No refolding of aggregated proteins required
  • Express up to 80 μg protein per reaction, due to CECF technology

Applications

  • Microgram-scale protein expression of functional disulfide-bonded proteins
  • Catalog #

    Size

    Package Content

    BR1400401

    24 rxn of 50 µl

    Reagents for coupled transcription/translation reactions


  • The RTS 100 E. coli Disulfide Kit provides an optimized combination of lysate, reagents, chaperones, and buffer systems to produce maximum yields of active multiple-disulfide-bonded protein in a CECF (continuous-exchange, cell-free) system.
    The kit employs several innovative technologies:

    • Coupled transcription/translation system; no mRNA isolation necessary
    • Optimized high-yield (HY) E. coli lysate
    • Redox buffer to maintain the system under oxidizing conditions, which enhances disulfide-bond formation
    • Disulfideisomerase for rearrangement of disulfide bonds, which enhances yields of correctly folded proteins
    • Increased amounts of GroE chaperones, which produce higher yields of soluble, properly folded proteins

    It utilizes CECF-technology, which prolongs the reaction time for at least 24 hours and therefore enhances the yield of produced proteins. Transcription, translation, and disulfide-bond formation take place in the 50µl reaction compartment of the reaction device. Substrates and energy components needed for a sustained reaction are continuously supplied via a semipermeable membrane. At the same time, potentially inhibitory reaction by-products are diluted via diffusion through the same membrane into the 1 ml feeding chamber. Depending on the particular protein, expression of up to 50 µg protein per 50 µl reaction can be obtained.