• RTS 100 E. coli LinTempGen Set, His-tag

RTS 100 E. coli LinTempGen Set, His-tag

  • Biotechrabbit
  • BR1400801
  • Na skladě
  • 10 400,00Kč
  • 10 200,00Kč

- OR -
Tento výrobek má minimální množství 1

Dopravné a balné

Transportní podmínky                                  cena se připočítává k položkám v objednávce

mražené na suchém ledu (dry ice)                   890 Kč bez DPH na zásilky v celkové ceně do 45 000 Kč bez DPH, nad tuto hodnotu je dopravné a balné zdarma


Features

  • Fast template generation without cloning
  • Contains all regulatory elements in optimal spacing
  • Create templates with N- or C-terminal 6xHis-tag
  • Primary PCR product can also be cloned into pIVEX vectors
  • For use with RTS 100 E. coli HY Kit

Applications

  • Generate linear templates for rapid screening and optimization of 6xHis-tagged protein expression

  • Catalog #

    Size

    Package Content

    BR1400801

    96 PCRs of 50 µl

    PCR reagents for linear template generation


  • The RTS E. coli Linear Template Set is designed to generate PCR products for use as templates for in vitro protein expression using the RTS 100 E. coli HY Kit. This method of generating DNA templates for protein expression avoids the time-consuming step of cloning into T7-expression plasmids.
    To generate transcriptionally active linear DNA fragments, two PCRs must be performed. In the first reaction, genespecific primers are used to add defined overlap regions to a desired gene sequence. The product of the first PCR is mixed with two flanking primers and DNA fragments that code for the T7-regulatory elements and a tag sequence. The first PCR product anneals with the added DNA fragments, and the 3' ends are extended. This final linear-expression construct is further amplified via the flanking primers.