illustra Ready-To-Go GenomiPhi V3 DNA Amplification Kits

25-6601-24

29013586

RTG GenomiPhi V3 Kit 24 rxns

25-6601-96

29013587

RTG GenomiPhi V3 Kit 96 rxns

illustra Ready-To-Go GenomiPhi V3 DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 12 to 20 μg yield from nanogram amounts of DNA sample.

• Representative amplification of the whole genome.
• Preformulated, predispensed, single-dose, lyophilized cakes for rapid reaction set-up and greater reproducibility.
• Quick and simple automation-friendly protocol; no thermal cycler required.
• Shipping and storage at room temperature provides easy access and reduced carbon footprint.
• Yields high-quality DNA (e.g. for high-throughput genotyping and DNA archival).

Description

illustra Ready-To-Go GenomiPhi V3 DNA Amplification Kit is part of the Phi29 DNA polymerase family of products from GE Healthcare. It contains all of the components necessary for whole genome amplification by isothermal strand displacement amplification. The reaction mixture is provided as predispensed, single-dose, lyophilized cakes in either strips of 8 tubes, or 96-well plates.

Amplification is highly uniform over the entire genome so that locus representation remains extremely close to the original DNA sample. Furthermore, amplification is carried out with very high fidelity due to Phi29 DNA polymerase proofreading activity. The average product length is over 10 kb.

The illustra Ready-To-Go GenomiPhi V3 DNA Amplification Kit procedure is quick, simple and does not require a thermocycler. A typical DNA yield of 12 to 20 µg DNA can be achieved in less than two hours from only 10 ng of genomic DNA input. The starting material for GenomiPhi reactions can be purified DNA from any commercial kit or homebrew method, or a nonpurified cell lysate may be used. Protocols for the amplification of DNA from blood lysates and dried blood spots are included in the manual.

GenomiPhi amplified DNA is suitable for various applications such as genotyping (SNP, STR, array CGH), cloning, sequencing, and DNA archival.