• 4X CAPITAL™ 1-Step qRT-PCR Green Master Mix

4X CAPITAL™ 1-Step qRT-PCR Green Master Mix

  • Biotechrabbit
  • BR0502300
  • In Stock
  • 4,186.00Kč

Available Options

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This product has a minimum quantity of 1

Storage temperature: Dry ice

Features

  • Convenient mix for quantification of RNA templates
  • Sensitive and specific amplification with rapid extension rate for early Ct values
  • Excellent linearity across a wide range of RNA dilution

Applications

  • One step qRT-PCR from mRNA, total RNA and viral RNA targets
  • For use with standard and fast qPCR platforms
  • BR0502301200 reactions of 20 µl1 ml CAPITAL qPCR Green Master Mix (1step)
    200 µl RTase with RNase Inhibitor

    BR05023021000 reactions of 20 µl5 × 1 ml CAPITAL qPCR Green Master Mix (1step)
    5 × 200 µl RTase with RNase Inhibitor

    BR05023034000 reactions of 20 µl4 kits BR0502302 CAPITAL 1-Step qRT-PCR Green Master Mix (1000 rxn each)
  • biotechrabbit CAPITAL™ qRT-PCR Green Mix allows sensitive and specific cDNA synthesis and qPCR in a single tube for quantifying mRNA, total RNA and viral RNA sequences. Extremely low-copy-number targets can be detected with high efficiency over several logs of template concentration.

    CAPITAL qRT-PCR Green Mix uses proprietary reverse transcriptase technology and buffer chemistry for efficient cDNA synthesis and QPCR in a single tube. To enable the use of the kit on qPCR platforms with different reference dye concentration requirements, three kit formats are available: a one-step kit containing no ROX, as well as LRox and HRox versions containing ROX in the corresponding concentratios.

     

    Info: Recommended annealing temperature is 2°C above primer Tm (use gradient PCR to optimize the annealing temperature).