GenUP™ Exo SAP Kit
- Biotechrabbit
- BR0701801
- In Stock
-
1,862.00Kč
Available Options
- OR -
This product has a minimum quantity of 1
Storage temperature: Dry ice
Features
- Fast 15 min PCR cleanup without the need for spin columns
- 100% recovery — no loss of PCR product
- Simple process — just add Exonuclease 1 and Shrimp Alkaline Phosphatase to PCR mix
- Easy scalability and automation
Applications
- PCR cleanup prior to sequencing or genotyping
- Removal of nucleotides and primers from PCR reaction
biotechrabbit™ GenUP Exo SAP Kit is designed for quickly and easily cleaning up PCR amplification reactions, enabling their direct use in sequencing or genotyping.
The kit provides a combination of Shrimp Alkaline Phosphatase (SAP) and Exonuclease 1 (ExoI) for removing nucleotides and primers. Double-stranded DNA is not affected, ensuring 100% recovery of PCR products.
Both enzymes are active in PCR buffers and completely inactivated by heating at 80°C: no further processing is required. Shrimp Alkaline Phosphatase dephosphorylates nucleotides and primers, and the 3' → 5' exonuclease activity of Exonuclease 1 degrades single-stranded primers.
| Catalog # | Size | Package Content | ||
|---|---|---|---|---|
| BR0701801 | 100 reactions | 200 µl ExoI-SAP | ||
| BR0701802 | 500 reactions | 1 ml ExoI-SAP | ||
| BR0701803 | 2000 reactions | 4 kits BR0701802 GenUP™ Exo SAP Kit (500 rxn each) |