uMRT Direct RNA-Seq Kit
- RNAConnect
- R1008-X
- In Stock
-
18,825.00Kč
Available Options
Get longer reads, detect more genes and isoforms, and experience improved modification signal-to-noise from your direct RNA-Seq. The UltraMarathonRT® (uMRT) Direct RNA-Seq Kit pairs the helicase-driven processivity of uMRT with the Oxford Nanopore Technologies® (SQK-RNA004) workflow.
Why Choose This Kit
- Detect more genes, isoforms, and longer, more contiguous native RNA reads
High processivity at low temperature preserves RNA integrity and reduces early terminations and read truncation. - Dramatically improved detection of long transcripts
Retains long RNAs that regularly degrade at high temperatures (e.g., >50°C). - Generate Higher cDNA yields from your Sample
uMRT’s optimized chemistry delivers higher cDNA yields for Direct RNA-Seq. - Drop-in for ONT workflows
Fits naturally into the Direct RNA-Seq protocol; no changes to downstream ligation, loading, or basecalling. - Convenience & Cost-Savings
One box contains everything you need to pair with ONT’s SQK-RNA004, eliminating the hassle and reducing the cost of ordering multiple reagents from different vendors.
Applications
- RNA modification mapping
- Isoform and UTR profiling directly on native RNA
- Viral and host–pathogen transcriptomics
- Long noncoding RNAs and repetitive/high-GC regions
UltraMarathonRT Direct RNA-Seq Kit Components:
- UltraMarathonRT® (20 U/μL)
- 2x RT Reaction Buffer
- dNTP Mix (10 mM each)
- RNA Conditioner
- 5x Ligation Buffer Q
- DNA Ligase (2,000,000 U/mL)
- RNase Inhibitor (40 U/μL)
- Quenching Buffer
- Nuclease-free Water
Reagents Required but Not Supplied:
- Oxford Nanopore Technologies® (ONT) SQK-RNA004 or SQK-RNA004-XL Direct RNA Sequencing kit.
- ONT MinION®/GridION® RNA Flow Cell (FLO-MIN004RA) or PromethION® RNA Flow Cell (FLO-PRO004RA).
- RNA Purification Beads.
- Qubit dsDNA HS Assay Kit (ThermoFisher, Q32851).
- RNA template: 500 ng - 1 μg total cellular RNA or 300 ng of poly (A)+ RNA is recommended for each reaction.
Product Information:
Catalog# R1008S (6 reactions), R1008M (48 reactions)
Storage Conditions: For short duration of storage (< 6 month), UltraMarathonRT can be stored in a -20℃ freezer. -80℃ is recommended for long-term storage. All other kit components are stable in a -20℃ freezer for long-term storage. Note that UltraMarathonRT can endure 10 freeze-thaw cycles without losing noticeable activity. After 20 freeze-thaw cycles, UltraMarathonRT maintains 90% of its original activity.
For Research Use Only.
