4X CAPITAL™ 1-Step qRT-PCR Probe Master Mix Plus

Storage temperature: Dry ice

4X CAPITAL™ 1-Step qRT-PCR Probe Master Mix Plus

4X CAPITAL qRT-PCR Probe Master Mix UDG • 20X RTase with RNase Inhibitor

R0502801 200 rxns of 20 µl 

BR0502802 1,000 rxns of 20 µl

BR0502803 4,000 rxns of 20 µl

FEATURES •

Prevents carryover contamination with UDG and dUTP for reliable RNA quantification • Integrates reverse transcription and PCR amplification in one tube, minimizing sample handling • Maintains enzyme activity and stability throughout thermal cycling

APPLICATION

Critical for comparing mRNA levels under various conditions • Effectively identifies RNA viruses in health-related screenings • Supports studies on RNA roles in disease and therapy

DESCRIPTION

biotechrabbit™ CAPITAL™ 1-Step qRT-PCR Probe Master Mix Plus is a state-of-the-art solution optimized for quantitative reverse transcription PCR (qRT-PCR). This advanced mix integrates reverse transcriptase, Taq DNA polymerase, Uracil-DNA Glycosylase (UDG), and dUTP in a single, ready-to-use formulation, making it ideal for accurate RNA target detection and gene expression analysis. The inclusion of UDG and dUTP enhances the mix's capability to prevent carryover contamination from previous PCR reactions. UDG excises uracil residues incorporated into DNA during PCR, and dUTP ensures that uracil-containing DNA is used, marking any potential contaminant for degradation. This dual approach significantly reduces the likelihood of amplifying carryover, thus improving the reliability of your results.